Lab Publications (2012-Today)

Restoration of TET2 Function Blocks Aberrant Self-Renewal and Leukemia Progression

Cimmino L, Dolgalev I, Wang Y, Yoshimi A, Martin GH, Wang J, Ng V, Xia B, Witkowski MT, Mitchell-Flack M, Grillo I, Bakogianni S, Ndiaye-Lobry D, Martín MT, Guillamot M, Banh RS, Xu M, Figueroa ME, Dickins RA, Abdel-Wahab O, Park CY, Tsirigos A, Neel BG, Aifantis I. Restoration of TET2 Function Blocks Aberrant Self-Renewal and Leukemia Progression. Cell. 2017 Aug 16 DOI: 10.1016/j.cell.2017.07.032, PMID: 28823558

Opposing Functions of H2BK120 Ubiquitylation and H3K79 Methylation in the Regulation of Pluripotency by the Paf1 Complex

Alexandros Strikoudis, Charalampos Lazaris, Panagiotis Ntziachristos, Aristotelis Tsirigos and Iannis Aifantis. Opposing functions of H2BK120 ubiquitylation and H3K79 methylation in the regulation of pluripotency by the Paf1 complex. Cell Cycle 2017, 10.1080/15384101.2017.1295194.

HiC-bench: comprehensive and reproducible Hi-C data analysis designed for parameter exploration and benchmarking

Charalampos Lazaris, Stephen Kelly, Panagiotis Ntziachristos, Iannis Aifantis & Aristotelis Tsirigos. HiC-bench: comprehensive and reproducible Hi-C data analysis designed for parameter exploration and benchmarking. BMC Genomics 2017 18:22. doi: 10.1186/s12864-016-3387-6

MED12 Regulates HSC-Specific Enhancers Independently of Mediator Kinase Activity to Control Hematopoiesis

Beatriz Aranda-Orgilles, Ricardo Saldaña-Meyer, Eric Wang, Eirini Trompouki, Anne Fassl, Stephanie Lau, Jasper Mullenders, Pedro P. Rocha, Ramya Raviram, María Guillamot, María Sánchez-Díaz, Kun Wang, Clarisse Kayembe, Nan Zhang, Leonela Amoasii, Avik Choudhuri, Jane A. Skok, Markus Schober, Danny Reinberg, Piotr Sicinski, Heinrich Schrewe, et al.
Cell Stem Cell, Volume 19, Issue 6, 1 December 2016, Pages 784-799

Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a.

Alexandros Strikoudis, Charalampos Lazaris, Thomas Trimarchi, Antonio L. Galvao Neto, Yan Yang, Panagiotis Ntziachristos, Scott Rothbart, Shannon Buckley, Igor Dolgalev, Matthias Stadtfeld, Brian D. Strahl, Brian D. Dynlacht, Aristotelis Tsirigos & Iannis Aifantis. Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a. Nature Cell Biology 18, 1127–1138 (2016) doi:10.1038/ncb3424.

The ubiquitin ligase Huwe1 regulates the maintenance and lymphoid commitment of hematopoietic stem cells.

Bryan King, Francesco Boccalatte, Kelly Moran-Crusio, Elmar Wolf, Jingjing Wang, Clarisse Kayembe, Charalampos Lazaris, Xiaofeng Yu, Beatriz Aranda-Orgilles, Anna Lasorella & Iannis Aifantis. The ubiquitin ligase Huwe1 regulates the maintenance and lymphoid commitment of hematopoietic stem cells. Nature Immunology 17, 1312–1321 (2016) doi:10.1038/ni.3559.

Emerging Concepts of Epigenetic Dysregulation in Hematological Malignancies.

Ntziachristos P, Abdel-Wahab O, Aifantis I.: Emerging concepts of epigenetic dysregulation in hematological malignancies. Nature Immunology 2016, 01 August.

The Impact of DNA Methylation in Hematopoietic Malignancies.

Guillamot M, Cimmino L, Aifantis I: The Impact of DNA Methylation in Hematopoietic Malignancies. Trends in Cancer 2016, 2:70–83.

Emerging roles for the FBXW7 ubiquitin ligase in leukemia and beyond.

N. Kourtis, A. Strikoudis, and I. Aifantis, “Emerging roles for the FBXW7 ubiquitin ligase in leukemia and beyond,”
Current Opinion in Cell Biology, vol. 37, pp. 28–34, Dec. 2015.

The CUL4-DDB1 ubiquitin ligase complex controls adult and embryonic stem cell differentiation and homeostasis.

Gao J, Buckley SM, Cimmino L, Guillamot M, Strikoudis A, Cang Y, Goff SP, Aifantis I. The CUL4-DDB1 ubiquitin ligase complex controls adult and embryonic stem cell differentiation and homeostasis.
Elife. 2015 Nov 27;4. pii: e07539. doi: 10.7554/eLife.07539. PMID: 26613412

Cohesin loss alters adult hematopoietic stem cell homeostasis, leading to myeloproliferative neoplasms.

Jasper Mullenders, Beatriz Aranda-Orgilles, Priscillia Lhoumaud, Matthew Keller, Juhee Pae, Kun Wang, Clarisse Kayembe, Pedro P. Rocha, Ramya Raviram, Yixiao Gong, Prem K. Premsrirut, Aristotelis Tsirigos, Richard Bonneau, Jane A. Skok, Luisa Cimmino, Daniela Hoehn and Iannis Aifantis. Cohesin loss alters adult hematopoietic stem cell homeostasis, leading to myeloproliferative neoplasms.
JEM 212(11):1833-1850, doi: 10.1084/jem.20151323.

CXCL12-Producing Vascular Endothelial Niches Control Acute T Cell Leukemia Maintenance.

Pitt LA, Tikhonova AN, Hu H, Trimarchi T, King B, Gong Y, Sanchez-Martin M, Tsirigos A, Littman DR, Ferrando AA, Morrison SJ, Fooksman DR, Aifantis I, Schwab SR. CXCL12-Producing Vascular Endothelial Niches Control Acute T Cell Leukemia Maintenance.
Cancer Cell. 2015 Jun 8;27(6):755-68. doi: 10.1016/j.ccell.2015.05.002. PubMed PMID: 26058075.

TET1 is a tumor suppressor of hematopoietic malignancy

Cimmino L, Dawlaty MM, Ndiaye-Lobry D, Yap YS, Bakogianni S, Yu Y, Bhattacharyya S, Shaknovich R, Geng H, Lobry C, Mullenders J, King B, Trimarchi T, Aranda-Orgilles B, Liu C, Shen S, Verma AK, Jaenisch R, Aifantis I. TET1 is a tumor suppressor of hematopoietic malignancy. Nat Immunol. 2015 Jun;16(6):653-62. doi: 10.1038/ni.3148. Epub 2015 Apr 13.
PubMed PMID: 25867473.

FBXW7 modulates cellular stress response and metastatic potential through HSF1 post-translational modification.

Nikos Kourtis, Rana S. Moubarak, Beatriz Aranda-Orgilles, Kevin Lui, Iraz T. Aydin, Thomas Trimarchi, Farbod Darvishian, Christine Salvaggio, Judy Zhong, Kamala Bhatt, Emily I. Chen, Julide T. Celebi, Charalampos Lazaris, Aristotelis Tsirigos, Iman Osman, Eva Hernando & Iannis Aifantis

Nature Cell Biology 17, 322–332 (2015) | doi:10.1038/ncb3121

Contrasting roles of histone 3 lysine 27 demethylases in acute lymphoblastic leukaemia.

Panagiotis Ntziachristos, Aristotelis Tsirigos, G. Grant Welstead, Thomas Trimarchi, Sofia Bakogianni, Luyao Xu, Evangelia Loizou, Linda Holmfeldt, Alexandros Strikoudis, Bryan King, Jasper Mullenders, Jared Becksfort, Jelena Nedjic, Elisabeth Paietta, Martin S. Tallman, Jacob M. Rowe, Giovanni Tonon, Takashi Satoh, Laurens Kruidenier, Rab Prinjha, Shizuo Akira, Pieter Van Vlierberghe, Adolfo A. Ferrando, Rudolf Jaenisch, Charles G. Mullighan & Iannis Aifantis.
Nature. 2014 Aug 17

Genome-wide Mapping and Characterization of Notch-Regulated Long Noncoding RNAs in Acute Leukemia.

Trimarchi T, Bilal E, Ntziachristos P, Fabbri G, Dalla-Favera R, Tsirigos A, Aifantis I.
Cell. 2014 Jul 31

Regulation of stem cell function by protein ubiquitylation.

Strikoudis A, Guillamot M, Aifantis I.
EMBO Rep. 2014

From fly wings to targeted cancer therapies: a centennial for notch signaling.

Ntziachristos P, Lim JS, Sage J, Aifantis I.
Cancer Cell. 2014 Mar 17

Notch signaling: switching an oncogene to a tumor suppressor.

Lobry C, Oh P, Mansour MR, Look AT, Aifantis I.
Blood. 2014 Apr 17

Deletion of Asxl1 results in myelodysplasia and severe developmental defects in vivo.

Abdel-Wahab O, Gao J, Adli M, Dey A, Trimarchi T, Chung YR, Kuscu C, Hricik T, Ndiaye-Lobry D, Lafave LM, Koche R, Shih AH, Guryanova OA, Kim E, Li S, Pandey S, Shin JY, Telis L, Liu J, Bhatt PK, Monette S, Zhao X, Mason CE, Park CY, Bernstein BE, Aifantis I, Levine RL.

J Exp Med. 2013 Nov 18

The RAG2 C-terminus and ATM protect genome integrity by controlling antigen receptor gene cleavage.

Chaumeil J, Micsinai M, Ntziachristos P, Roth DB, Aifantis I, Kluger Y, Deriano L, Skok JA.

Nat Commun. 2013

A new player SETs in myeloid malignancy.

Trimarchi T, Ntziachristos P, Aifantis I.

Nat Genet. 2013 Aug

The ubiquitin ligase FBXW7 modulates leukemia-initiating cell activity by regulating MYC stability.

King B, Trimarchi T, Reavie L, Xu L, Mullenders J, Ntziachristos P, Aranda-Orgilles B, Perez-Garcia A, Shi J, Vakoc C, Sandy P, Shen SS, Ferrando A, Aifantis I.

Cell. 2013 Jun 20

In vivo mapping of notch pathway activity in normal and stress hematopoiesis.

Oh P, Lobry C, Gao J, Tikhonova A, Loizou E, Manent J, van Handel B, Ibrahim S, Greve J, Mikkola H, Artavanis-Tsakonas S, Aifantis I.

Cell Stem Cell. 2013 Aug 1

Mechanisms of epigenetic regulation of leukemia onset and progression.

Ntziachristos P, Mullenders J, Trimarchi T, Aifantis I.

Adv Immunol. 2013

 Regulation of c-Myc ubiquitination controls chronic myelogenous leukemia initiation and progression.

Reavie L, Buckley SM, Loizou E, Takeishi S, Aranda-Orgilles B, Ndiaye-Lobry D, Abdel-Wahab O, Ibrahim S, Nakayama KI, Aifantis I.

Cancer Cell. 2013 Mar 18

Higher-order looping and nuclear organization of Tcra facilitate targeted rag cleavage and regulated rearrangement in recombination centers.

Chaumeil J, Micsinai M, Ntziachristos P, Deriano L, Wang JM, Ji Y, Nora EP, Rodesch MJ, Jeddeloh JA, Aifantis I, Kluger Y, Schatz DG, Skok JA.

Cell Rep. 2013 Feb 21

Distinct TCR signaling pathways drive proliferation and cytokine production in T cells.

Guy CS, Vignali KM, Temirov J, Bettini ML, Overacre AE, Smeltzer M, Zhang H, Huppa JB, Tsai YH, Lobry C, Xie J, Dempsey PJ, Crawford HC, Aifantis I, Davis MM, Vignali DA.

Nat Immunol. 2013 Mar

Notch pathway activation targets AML-initiating cell homeostasis and differentiation.

Lobry C, Ntziachristos P, Ndiaye-Lobry D, Oh P, Cimmino L, Zhu N, Araldi E, Hu W, Freund J, Abdel-Wahab O, Ibrahim S, Skokos D, Armstrong SA, Levine RL, Park CY, Aifantis I.

J Exp Med. 2013 Feb 11

Regulation of pluripotency and cellular reprogramming by the ubiquitin-proteasome system.

Buckley SM, Aranda-Orgilles B, Strikoudis A, Apostolou E, Loizou E, Moran-Crusio K, Farnsworth CL, Koller AA, Dasgupta R, Silva JC, Stadtfeld M, Hochedlinger K, Chen EI, Aifantis I.

Cell Stem Cell. 2012 Dec 7

The taming of the NF-κB: PP4R1 navigates while PP4c dephosphorylates.

Tikhonova A, Aifantis I.

Immunity. 2012 Oct 19